Glossary

T

Tm (Melting Temperature)

Melting temperature is the temperature at which 50% of the limiting reagent is bound in the designated complex given that there are no intermediate complexes.  Therefore, in the case of a Target-Probe heterodimer where the target has a concentration of 1 uM and the probe has a concentration of 2 uM, then the melting temperature would be the temperature at which half of the target is bound to the probe assuming that the target can only exist as a random coil or as part of the heterodimer AND assuming that the probe can also exist only as a random coil or as part of the heterodimer.  If other species and intermediates are considered, melting temperatures may be determined using effective Tm or Net Tm.  

Traceback Structure File (TBS)

Each TBS file contains base pairing information about a single species necessary by Visual OMP to draw a graphical representation.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z