21) Changing the right nucleotide to make Mutant Allele
Go to the nucleotide of the variation.
Change it to make the mutant allele. In this case I need to change the G at nucleotide 300 (outlined with red circle) to a T to make the mutant allele (IUPAC code K means either G or T).
Now we are ready to simulate the two targets together with both beacons:
go back to the sequences tab
check the Experiment Conditions and Design Settings tabs
save the experiment and run the simulation
