8) Determination of a primer and probe location
A secondary structure of the target is now visible on the screen
This structure represents the optimal, or most likely secondary structure of our target
Cycle through the optimal and suboptimal structures by clicking on the buttons outlined with a red circle (two grey buttons at the bottom right of the secondary structure form)
It will become evident that the secondary structure free range of nucleotides 110-160 represents a good location to design a probe in and a primer pair flanking this probe
Now that we know where we would like to design probes and primers, let's go to the design tab.
First close up the secondary structure graphic
Then close the Results Summary
Click on the "Probe/Primer Design" Tab on the Sequence Form
