Scorpio Tutorial:

This tutorial will introduce a new user on how to start, run, and view the results of a simple scorpion design and analysis, using step by step instructions.   The tutorial will follow the design of an ARMS Scorpion primer with hints on how to design other primers (descriptions exist on the next 2 pages).  While reading the tutorial, it may be useful to attempt to duplicate the steps on a sequence of familiarity.

The three concepts that this tutorial will cover are General Overview, Design of Scorpions and Analysis of Scorpions.  More specifically, these will demonstrate:

• General ideas and concepts

• How Scorpio works

• General Scorpion Concepts

• Starting the application

• Open vs. Closed Scorpions

• Basic Design Form

• How to work with weights

• Temperatures and Concentrations

• Additional Oligos

• ARMS, ASO and QPCR

• Advanced Design Form

• Designing Scorpions

• Overview of Output

• Thermodynamics

• Analyzing Scorpion Designs

• Input

• Additional Oligos

• Output

• Simulating Scorpions

• Numerical Analysis

• Secondary Structure

• Unextended

If you are ready to get started, press the "next" button below.